| Post Name | Microbiologist(bacteriologist) |
|---|---|
| Question Paper Code | 096/2024 |
| Category Code | 411/2023, 412/2023 |
| Date of Test | 29 July 2024 |
| Medium of Question | ENGLISH |
| Exam Type | OMR Examination |
| Total Questions | 100 |
1
Which of the following is a common method for disinfecting drinking water?
Answer
C. Chlorination
2
The process of removing or killing all microorganisms in a material or on an object is known
as :
Answer
B. Sterilization
3
Which type of microscopy enhances the contrast of transparent and colourless objects without
staining?
Answer
C. Phase-contrast microscopy
4
The resolving power of a light microscope is primarily determined by :
Answer
B. Numerical aperture of the objectives lens
5
In continuous culture, which device maintains bacterial cultures in a state of exponential
growth?
Answer
B. Chemostat
6
Who first provided evidence that microorganisms can cause disease in humans?
Answer
A. Robert Koch
7
Which culture method is used to determine the antibiotic susceptibility of bacteria?
Answer
Question Cancelled
8
What is the purpose of using a selective medium in microbiological culture?
Answer
D. To inhibit the growth of certain organisms while allowing others to grow
9
Which medium is specifically designed for the isolation of Gram-positive bacteria, inhibiting
Gram-negative bacteria?
Answer
B. Mannitol salt agar
10
Which type of wastewater treatment involves the use of microorganisms to decompose
organic matter?
Answer
B. Secondary treatment
11
Which of the following is the most effective method to prevent the spread of many infectious
diseases?
Answer
D. Handwashing with soap and water
12
Which of the following is an example of a vector-borne disease?
Answer
C. Malaria
13
Which waterborne disease is caused by a protozoan parasite and can lead to severe
diarrhoea?
Answer
C. Giardiasis
14
Which of the following antibiotics is effective against a wide range of both Gram-positive and
Gram-negative bacteria?
Answer
C. Ciprofloxacin
15
Which of the following is a mechanism by which bacteria develop resistance to antibiotics?
Answer
B. Efflux pumps that remove the antibiotic from the cell
16
Which class of antibiotics inhibits bacterial protein synthesis by binding to the 50S ribosomal
subunit?
Answer
C. Macrolides
17
In fluorescence microscopy, the fluorescent dyes used are excited by which type of light?
Answer
B. Ultraviolet light
18
The Gram staining technique differentiates bacteria based on the composition of their :
Answer
C. Cell wall
19
Which class of antibiotics inhibits bacterial protein synthesis by binding to the 50S ribosomal
subunit?
Answer
C. Macrolides
20
Which method is used to increase the contrast of specimens in TEM?
Answer
A. Coating with heavy metals
21
Which of the following is used in cold sterilisation?
Answer
D. Gamma rays
22
Toxins are produced by bacteria in :
Answer
C. Stationary phase
23
Organelle involved in bacterial respiration is :
Answer
C. Mesosome
24
The enzyme which is absent in anaerobes is :
Answer
Question Cancelled
25
Enhancement of virulence in bacteria is called :
Answer
C. Exaltation
26
A population of bacteria derived by binary fission from a single cell is called :
Answer
B. Clone
27
Organisms which prefer cold environments with optimum growth temperature of 15°C or
below are :
Answer
C. Psychrophilic
28
Culture Media used for Anaerobic organisms :
Answer
B. Robertson’s Cooked meat broth
29
Capnophilic bacteria require –––––––––––– for growth and multiplication.
Answer
D. Carbon dioxide
30
Total energy production from One Glucose molecule is :
Answer
Question Cancelled
31
T lymphocytes are identified by :
Answer
A. Rosette formation with sheep RBC
32
The immunoglobulin class is determined by :
Answer
A. Heavy chain isotype
33
Type of immune response in transplant rejection is :
Answer
B. Type II
34
Grafts transplanted between identical twins :
Answer
C. Isograft
35
Which one of the following could function both as central and peripheral lymphoid organ?
Answer
Question Cancelled
36
Antigen recognition on the surface of the Antigen presenting cell is by :
Answer
Question Cancelled
37
When a soluble antigen combines with its antibody in the presence of electrolytes at suitable
temperature and pH, the reaction is :
Answer
B. Precipitation
38
Which blood group antibody is IgG class?
Answer
D. Anti Rh
39
Which complement fragment is called C3 convertase : in Complement cascade?
Answer
B. C14b2a
40
A substance reacts with an antibody but no antibody formation in its native state is called :
Answer
D. Hapten
41
A method of horizontal gene transfer among prokaryotes in which DNA is transferred from
one cell to another via a replicating virus :
Answer
A. Transduction
42
A DNA gene synthesized from an RNA template is :
Answer
A. Complementary DNA
43
A type of electrophoresis which is extensively used for the quantification and analysis of
proteins?
Answer
C. 2D electrophoresis
44
Auxotrophic mutants are referred to as :
Answer
C. Nutritional
45
Beer Lambert’s law gives the relation between which of the following?
Answer
D. Energy absorption and concentration
46
Choose the false statement concerning vectors in recombinant DNA technology :
Answer
Question Cancelled
47
DNA repair system that is used to remove pyrimidine dimers formed by UV radiation :
Answer
A. Nucleotide excision Repair
48
–––––––––––– is used as a media for density gradient.
Answer
B. Ficoll
49
Ethidium bromide is used in gel electrophoresis :
Answer
A. Stain DNA and make it visible under UV light
50
In a native PAGE, proteins are separated on the basis of :
Answer
D. Net charge and size
51
Light source used in Uv-Vis spectroscopy :
Answer
Question Cancelled
52
Mode of action of β-Lactamases in rendering penicillin resistance is by :
Answer
B. Degrading the antibiotic
53
One cell takes DNA from the other and inserts it into its chromosome despite the fact that
the two cells are completely unrelated. The procedure is :
Answer
A. Horizontal gene transfer
54
Plasmids are :
Answer
C. Accessory genetic information
55
The study of genomes recovered from natural samples without first isolating members of the
microbial community and growing them in pure cultures :
Answer
D. Metagenomics
56
The process used in the laboratory to produce millions of copies of DNA is :
Answer
C. Polymerase Chain Reaction (PCR)
57
Transposons are :
Answer
D. All of the above
A 9 096/2024
58
Which of the following is not a type of centrifugation?
Answer
B. Microfiltration
59
DNA fingerprinting consists of identifying individuals or organisms by their unique :
Answer
C. Restriction enzyme fragments of DNA molecules
60
Which statement is not true regarding difference between prokaryotes and eukaryotes?
Answer
B. Polyribosomes are present in prokaryotes but not by eukaryotes
61
Growth of culture without the presence of added free water is :
Answer
B. Solid state fermentation
62
Phenylacetic acid is added as a precursor to maximize the production of antibiotic :
Answer
A. Penicillin G
63
The amino acids lysine and –––––––––– are used in the food industry as nutritional
supplements in bread products and as flavor enhancing compounds such as monosodium
glutamate.
Answer
A. Glutamic acid
64
Citric acid is produced by the microorganism :
Answer
B. Aspergillus niger
65
Antibiotics and mycotoxins falls under which category of microbial products :
Answer
B. Secondary metabolites
66
In continuous culture techniques using chemostat, cell output is improved by maintain
microorganisms in –––––––––– phase.
Answer
B. Logarithmic
67
α -amylase is industrially produced from the microorganism :
Answer
A. Bacillus subtilis
68
An industrial strain or microorganism should ideally exhibit :
Answer
D. All of these
69
–––––––––– are used as nitrogen source in fermentation media.
Answer
D. All of these
70
–––––––––– have been particularly useful for biomass production for animal feed.
Answer
C. Fluidized bed reactor
71
Benzoate shows greatest antimicrobial activity at –––––––––– pH.
Answer
A. 4
72
Homofermentative lactic acid bacteria lacks the enzyme :
Answer
B. Phosphoketolase
73
Yoghurt is produced using a mixed culture of Streptococcus thermophiles and Lactobacillus
bulgaricus in the ratio :
Answer
Question Cancelled
74
Propionibacterium shermanii is added to –––––––––– cheese for flavour development and eye
formation.
Answer
D. Swiss
75
–––––––––– is a staple food of West Africa prepared by fermenting root of cassava plant.
Answer
A. Gari
76
Sake is an alcoholic beverage prepared from :
Answer
A. Steamed rice
77
‘Black leg’ in potatoes is caused by –––––––––– sp.
Answer
A. Erwinia
78
Cereolysin is a thiol activated toxin produced by :
Answer
B. Bacillus cereus
79
Food borne outbreaks associated with egg and poultry products are generally caused by :
Answer
A. Salmonella
80
Alarm water content is the water content that should not be exceeded if –––––––––– is to be
avoided.
Answer
B. Mold growth
81
Bacillus anthracis was isolated by :
Answer
B. Robert Koch
82
In which organ does Salmonella Typhi exist in carriers?
Answer
C. Gall bladder
83
Warthin-Starry silver staining is used for demonstration of :
Answer
A. Leptospira
84
Urea breath test is used to diagnose :
Answer
C. Helicobacter pylori
85
Toxin produced by certain strains of both E.coli and Shigella :
Answer
C. Shiga toxin
86
In its action, Diphtheria toxin resembles the toxin of :
Answer
D. Pseudomonas aeruginosa
87
The most common agent causing respiratory infection in cystic fibrosis patients :
Answer
A. Pseudomonas aeruginosa
88
Appearance of Bordetella in Gram’s smear resembles :
Answer
C. Thumb print
89
Which of the following pneumococcal vaccine is not recommended for children under 2 years
of age?
Answer
B. 23 valent polysaccharide vaccine
90
Reduction of disease incidence, prevalence, morbidity or mortality to a locally acceptable level
as a result of deliberate efforts is known as :
Answer
C. Control
91
In Eijkman test, MacConkey broth tubes are incubated at :
Answer
A. 44°C
92
Trickling filter method is used in which of the following :
Answer
C. Secondary sewage treatment
93
Ground water include all except :
Answer
D. Lakes
94
Chlorine demand is measured by :
Answer
A. Horrock’s apparatus
95
Evaluation of the quality of air can be performed by all the following method except :
Answer
D. Sweep plate method
96
Which of the following method is used for detection of endotoxin in water?
Answer
C. Limulus amebocyte lysate assay
97
HEPA filter used in biosafety cabinet is :
Answer
D. High efficiency particulate air
98
Which of the following is the heart of the activated sludge process?
Answer
B. Aeration tank
99
Which of the following is not used to express the strength of sewage?
Answer
A. E.coli count
100
Aerosol transmission occurs in :
Answer
A. Mycobacterium tuberculosis
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